Carlo Emanuele Villa

This paper creates a molecular atlas of endocrine signaling in human neural organoids by perturbing seven hormonal pathways and analyzing their effects on neurodevelopment.

MintFlow is a generative AI algorithm that predicts how tissue perturbations reprogram cell states in disease, revealing novel cell populations and therapeutic targets in three human conditions.

The paper uses combinatorial CRISPR-Cas9 perturbations in brain organoids to uncover regulatory hierarchies of human-specific genes involved in cortical evolution.

This paper develops a novel integration method for highly heterogeneous single-cell transcriptomic data and applies it to high-grade serous ovarian cancer to generate a reference atlas, revealing disease progression and treatment-related cell states.

This study identifies a human-specific CHD2 regulatory variant that influences cortical development tempo via lysosomal homeostasis, showing therapeutic potential through lysosomal restoration.

The paper develops and validates multiplexing strategies for brain organoids combined with single cell RNA-seq to study neurodevelopmental trajectories across multiple genetic lines.

This paper integrates 36 single-cell transcriptomics datasets from human neural organoids into a unified atlas to assess cell type coverage and compare organoid protocols with primary brain tissue.

This study shows that GTF2I dosage affects neuronal differentiation and social behavior in 7q11.23 neurodevelopmental disorders using patient-derived organoids and mouse models.

This study shows that CHD8 haploinsufficiency disrupts human cortical development by altering neuron generation timing, leading to macrocephaly-like organoid enlargement and cell-type specific molecular defects.

This study uses human cortical organoids to show that GSK3 inhibition affects progenitor proliferation and tissue architecture, with specific impacts on outer radial glia and astrogenic lineage.