Author

Oliviero Leonardi

University of MilanORCID 0009-0002-0702-4366Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Neuroscience· 2 Molecular Biology· 1 Systems Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 4 Research Focus:differential expression· 2 Research Focus:gene regulatory network inference· 2 Assay:ATAC-seq· 1 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1

Frequent Co-Authors

Alessandro VitrioloiD· 4 shared Cedric A. BoeckxiD· 4 shared Giuseppe TestaiD· 4 shared Filippo PrazzoliiD· 3 shared Carlo Emanuele VillaiD· 2 shared Davide AprileiD· 2 shared Davide CastaldiiD· 2 shared Juan MorianoiD· 2 shared

Activity by Year

2025-2026 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 42 mutual co-author links

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Focal author (center)Selected co-authorORCID co-authorUnlinked name

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4 shared papersNeuroscience

Alessandro VitrioloORCID

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4 papers

Recent·Most cited

Co-regulatory changes in Homo sapiens prefrontal cortex shape RNA-chromatin interactions

Alessandro Vitriolo, Oliviero Leonardi, Veronica Finazzi, Davide Castaldi, Lorenzo Basile, Filippo Prazzoli, Alice Lambolez, Kayoko Yasuzawa, Chi Wai Yip, Mitsuyoshi Murata, Aslıhan Karabacak Calviello, Rodrigo Pracana, Robert Lehmann, Jesper N. Tegner, Vincenzo Lagani, , Giuseppe Testa

The paper maps RNA-chromatin interactions in human prefrontal cortex, revealing co-regulatory changes linked to brain evolution and disease pathways.

bioRxiv · Mar 17, 2026 · PreprintMolecular Biology

Benchmarking cerebellar organoids to model autism spectrum disorder and human brain evolution

Davide Aprile, Oliviero Leonardi, Alessia Petrella, Davide Castaldi, Lorenza Culotta, Cristina Cheroni, Alessandro Valente, Matteo Bonfanti, Alessandro Vitriolo, Juan Moriano, Filippo Mirabella, Amaia Tintori, Cedric A. Boeckx, Giuseppe Testa

The paper longitudinally profiles cerebellar organoids, benchmarks them against fetal data, and models mutations linked to autism and human brain evolution.

bioRxiv · May 14, 2025 · PreprintNeuroscience

Regulatory logic of human cortex evolution by combinatorial perturbations

Adrianos Skaros, Alessandro Vitriolo, Oliviero Leonardi, Veronica Finazzi, Marlene F. Pereira, Filippo Prazzoli, Sebastiano Trattaro, Juan Moriano, Daniele Capocefalo, Carlo Emanuele Villa, Michael Boettcher, Cedric A. Boeckx, Giuseppe Testa

The paper uses combinatorial CRISPR-Cas9 perturbations in brain organoids to uncover regulatory hierarchies of human-specific genes involved in cortical evolution.

bioRxiv · Apr 28, 2025 · PreprintSystems Biology

Human-Specific Regulation of CHD2 Controls the Tempo of Cortical Development through Lysosomal Homeostasis

Oliviero Leonardi, Elly Lewerissa, Davide Aprile, Tahsin Stefan Barakat, Lorenza Culotta, Ruizhi Deng, Linda Haazen, Katrin Linda, Marian Majoie, Annika Mordelt, Aurelio Ortale, Astrid R. Oudakker, Filippo Prazzoli, Sofía Puvogel, Nicky Scheefhals, , Nael Nadif Kasri

This study identifies a human-specific CHD2 regulatory variant that influences cortical development tempo via lysosomal homeostasis, showing therapeutic potential through lysosomal restoration.

bioRxiv · Jan 21, 2025 · PreprintNeuroscience