Mattheos A. G. Koffas

This paper improves synthetic transcription factors in yeast to enhance production of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) through engineered loop flexibility and stability.

The paper develops a dCas12a-based tool for programmable gene activation and repression in prokaryotes, demonstrating its utility in metabolic engineering.

This study optimizes Y. lipolytica for producing flavonoids and hydroxylated flavonoids by engineering metabolic pathways and enzyme modules.