Author

Zakary S. Singer

Columbia UniversityORCID 0000-0002-1907-3635Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:drug response / drug discovery· 3 Organism:bacteria (other)· 1 Research Focus:cell-cell communication· 1 Organism:E. coli· 1 Assay:imaging· 1 Organism:mouse· 1

Frequent Co-Authors

Tal DaninoiD· 4 shared Charles M. RiceiD· 2 shared Alexander MichelsiD· 1 shared Amy LuiD· 1 shared Audrey Lam· 1 shared Azra HasekiiD· 1 shared Courtney CokeriD· 1 shared Edward R. BallisteriD· 1 shared

Activity by Year

2019-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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4 shared papersSynthetic Biology

Tal DaninoORCID

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4 papers

Recent·Most cited

NanoPro - a Framework for Probiotically-Guided Nanovector Therapy

Alexander Michels, Azra Haseki, Myra Ashraf, Audrey Lam, Kailyn R Grant, Amy Lu, Rosa L. Vincent, Jongwon Im, Zakary S. Singer, Edward R. Ballister, Tal Danino

The paper introduces NanoPro, a nanovector therapy platform using probiotically-released tumor-binding tags to target antigenically indistinct tumors, demonstrating in vitro efficacy with high transduction rates.

bioRxiv · Oct 29, 2026 · PreprintSynthetic Biology

Engineered bacteria launch and control an oncolytic virus

Zakary S. Singer, Jonathan Pabón, Hsinyen Huang, William Y. Sun, Hongsheng Luo, Kailyn Rhyah Grant, Ijeoma Obi, Courtney Coker, Charles M. Rice, Tal Danino

This paper describes engineered bacteria that deliver a viral genome to initiate oncolytic infection in tumors, demonstrating bacterial control over viral replication.

Nature Biomedical Engineering · Aug 15, 2025Peer-reviewedSynthetic Biology

Early alphavirus replication dynamics in single cells reveal a passive basis for superinfection exclusion

Zakary S. Singer, Pradeep M. Ambrose, Tal Danino, Charles M. Rice

This study uses single-cell imaging to show alphavirus superinfection exclusion is a graded, bidirectional process rather than a binary one, revealing a passive mechanism.

Cell Systems · Jan 29, 2021Peer-reviewedSystems Biology

Rapid screening of engineered microbial therapies in a 3-D multicellular model

Tetsuhiro Harimoto, Zakary S. Singer, Oscar S. Velazquez, Joanna T. Zhang, Samuel Castro, Taylor E. Hinchliffe, William Mather, Tal Danino

The paper presents a 3-D multicellular model to rapidly screen engineered microbial therapies, identifying effective antitumor candidates and demonstrating scalability across microbial species.

Proceedings of the National Academy of Sciences · Apr 17, 2019Peer-reviewedSynthetic Biology