The paper introduces PhIAT, a method using PhiC31 integrase to efficiently integrate extrachromosomal arrays into C. elegans chromosomes, enabling stable genomic modifications.

Science Advances · Oct 7, 2026Peer-reviewedGenetics

Modular gene tagging in C. elegans

Erik M. Jørgensen, Adam Hefel, Kevin Kruse, Kaden Wall, Soren Jorgensen, Kam Ng, Ryan Stolley, Matthew S. Rich

The paper introduces PhIT, a recombinase-based method for modular protein tagging in C. elegans, using CRISPR to insert a landing pad and PhiC31 integrase for tag insertion.

bioRxiv · Jan 15, 2026 · PreprintGenomics

RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis

Adam Hefel, Masayoshi Honda, Nicholas M. Cronin, Kailey Harrell, Pooja Patel, Maria Spies, Sarit Smolikove

This study identifies unique roles of RPA complexes in C. elegans meiosis, showing RPA-2 is essential for replication and repair, while RPA-4 inhibits RAD-51 and promotes apoptosis.

Nucleic Acids Research · Dec 29, 2020Peer-reviewedGenetics

Single-molecule long-read sequencing reveals the chromatin basis of gene expression

Yunhao Wang, Anqi Wang, Zujun Liu, Andrew L. Thurman, Linda S. Powers, Meng Zou, Yue Zhao, Adam Hefel, Yunyi Li, Joseph Zabner, Kin Fai Au

The paper introduces MeSMLR-seq to study chromatin accessibility and nucleosome organization at the single-cell level in yeast, revealing correlations between chromatin states and gene transcription.

Genome Research · Aug 1, 2019Peer-reviewedBioinformatics