Marianna A. Tryfonidou

Recapitulating the notochord to nucleus pulposus transition from human mesendoderm progenitors in 3D

This study develops a 3D model to recapitulate the transition from human mesendoderm progenitors to nucleus pulposus-like cells using single-cell transcriptomics and spatial profiling.

Degenerated intervertebral disc environment impairs notochordal cell-derived extracellular vesicles release and their matrix anabolic effect

This study shows that degenerative disc conditions reduce notochordal cell-derived extracellular vesicles release and their ability to promote matrix production in nucleus pulposus cells.

Understanding the physiological behaviour of disc cells in an in vitro imitation of the healthy and degenerated disc niche

This study develops media formulations to mimic the physiological conditions of healthy and degenerate intervertebral discs and assesses their effects on cell viability and secretome.