The paper describes a low-cost, lab-assembled TFF system for EV isolation from diverse cell culture sources and evaluates its performance with and without SEC.

bioRxiv · Oct 6, 2026 · PreprintCell Biology

Multidimensional Cellular Micro-Compartments to Model Invasive Lobular Carcinoma Dormancy

Xilal Y. Rima, Sarmila Majumder, Divya S. Patel, Chunyu Hu, Hong Li, Xin Huang, Kim Truc Nguyen, Jacob Doon‐Ralls, Chiranth K. Nagaraj, Mangesh Dattu Hade, Setty M. Magaña, Eswar Shankar, Xiaoli Zhang, Daniel G. Stover, Bhuvaneswari Ramaswamy, Eduardo Reátegui

This study develops multidimensional in vitro models to investigate anti-estrogen resistance and dormancy in invasive lobular carcinoma, identifying p27Kip1 signaling as central to dormant phenotype.

Advanced Healthcare Materials · May 19, 2026Peer-reviewedBioengineering

Light-induced Extracellular Vesicle Adsorption

Colin L. Hisey, Xilal Y. Rima, Jacob Doon‐Ralls, Chiranth K. Nagaraj, Sophia Mayone, Kim Truc Nguyen, Sydney Wiggins, Kalpana Deepa Priya Dorayappan, Xin Huang, Mangesh Dattu Hade, Karuppaiyah Selvendiran, James N. Higginbotham, Oleg S. Tutanov, Jeffrey L. Franklin, Robert J. Coffey, , Eduardo Reátegui

This paper introduces LEVA, a label-free, high-resolution, tunable platform for EV adsorption and characterization, demonstrating its versatility with various EV sources and applications.

Nature Methods · Nov 18, 2025Peer-reviewedBioengineering

Human differentiated adipocytes can serve as surrogate mature adipocytes for adipocyte-derived extracellular vesicle analysis

Mangesh Dattu Hade, Bradley L. Butsch, Paola Loreto Palacio, Kim Truc Nguyen, Dharti Shantaram, Sabrena F. Noria, Stacy A. Brethauer, BRADLEY J. NEEDLEMAN, Willa A. Hsueh, Eduardo Reátegui, Setty M. Magaña

The paper shows that human differentiated adipocytes can produce EVs similar to mature adipocytes, providing a reliable alternative for studying adipocyte-derived extracellular vesicles in obesity research.

bioRxiv · Feb 5, 2025 · PreprintCell Biology

Novel multiparametric bulk and single extracellular vesicle pipeline for adipose cell-specific biomarker discovery in paired human biospecimens

Mangesh Dattu Hade, Jacelyn Greenwald, Paola Loreto Palacio, Kim Truc Nguyen, Dharti Shantaram, Bradley L. Butsch, Yongseok Kim, Sabrena F. Noria, Stacy A. Brethauer, BRADLEY J. NEEDLEMAN, Willa A. Hsueh, Vicki H. Wysocki, Eduardo Reátegui, Setty M. Magaña

The paper develops a multiparametric pipeline to isolate and characterize adipose-derived extracellular vesicles from human biospecimens, identifying cell-specific biomarkers and pathways associated with obesity.

bioRxiv · Apr 18, 2024 · PreprintMolecular Biology