Cesar L. Moreno

TriPort tangential flow filtration: a lab-assembled system for EV isolation across diverse cell-culture sources

The paper describes a low-cost, lab-assembled TFF system for EV isolation from diverse cell culture sources and evaluates its performance with and without SEC.

Proteolytic control of the SARS-CoV-2 furin cleavage site defines the phenotypic evolution from the pandemic to endemic state

The paper shows how SARS-CoV-2 variants, particularly Omicron, evolved proteolytic controls at the Spike furin cleavage site to enhance transmission while reducing lower respiratory tract pathogenicity.

FBXW11 Activity Regulates Radial Glial Expansion in Human Cerebral Organoids

The paper identifies FBXW11 as a negative regulator of radial glial expansion and neuronal maturation in human cerebral organoids through CRISPR screening and functional validation.

Replication of SARS-CoV-2 Omicron lineages is defined by TMPRSS2 use in environments where ACE2 is complexed with solute carriers SLC6A19 and SLC6A20.

This paper shows that SARS-CoV-2 Omicron lineages replicate using a specific ACE2 pool that does not complex with TMPRSS2, unlike pre-Omicron variants.

A chimeric viral platform for directed evolution in mammalian cells

This paper introduces PROTEUS, a chimeric viral platform for directed evolution in mammalian cells, demonstrating its ability to generate sensitive gene regulation tools and DNA damage-responsive nanobodies.

iPSC-Derived PSEN2 (N141I) Astrocytes and Microglia Exhibit a Primed Inflammatory Phenotype

This study differentiates and characterizes iPSC-derived astrocytes and microglia from PSEN2 (N141I) mutation carriers, showing a primed inflammatory phenotype with altered cytokine secretion and Aβ42 handling.

Characterization of cell-cell communication in COVID-19 patients

The paper characterizes cell-cell communication patterns in COVID-19 patients to identify differences between moderate and severe disease outcomes using single-cell RNA-seq data.