Vicki Hopper Wysocki

This study uses native mass spectrometry to show how PROTAC linker length and targeting ligand affect ternary complex structure and stability.

This study shows that Psi RNA binding promotes a conformational change in HIV-1 Gag from compact to extended, essential for viral particle assembly, supported by FRET and molecular dynamics simulations.

This study uses native mass spectrometry to reveal the half-site reactivity mechanism of Salmonella FraB, showing how substrate binding and conformational changes regulate catalytic activity in a homodimeric enzyme.

The paper develops SIDFold, a deep learning model that integrates SID-nMS data with AlphaFold to improve protein complex structure prediction accuracy.

This study determines the structure of Mgm101, a RAD52 homolog, and reveals its single-strand annealing mechanism using cryo-EM and mass spectrometry, showing it anneals ssDNAs on the same ring in cis.

This study investigates how ZW-1261, an FGBP-targeting inhibitor, disrupts HIV-1 capsid morphology by affecting pentameric subunits and core closure.

The paper improves protein complex structure prediction by integrating ion mobility mass spectrometry data with AlphaFold and Rosetta, achieving better RMSD values for many complexes.

This study investigates how phosphorylation of SARS-CoV-2 Np's SRR linker affects its RNA binding and packaging functions through biophysical and biochemical analyses.

The paper uses native mass spectrometry to study ATP binding to Rho, revealing binding to hexameric and lower-order complexes and super-stoichiometric binding at partially formed sites.

This study identifies a hetero-trimeric complex formed by Setdb1 and two Atf7IP copies that blocks Setdb1 nuclear export by competing with CRM1.

This study investigates nearest-neighbor cooperativity in TRAP by engineering dTRAP variants and using cryo-EM and thermodynamic analyses to reveal structural and thermodynamic mechanisms of ligand binding cooperativity.

The paper identifies topological rearrangements in the HerA-DUF complex that activate the anti-phage defense system, challenging the idea that oligomerization alone is sufficient for immune signaling.

This study uses multi-omics to analyze Salmonella's metabolic strategies in mice on different diets over time, identifying phase-specific gene expression linked to infection stages and therapeutic targets.

This paper introduces a new native proteomics technique using nCZE-MS to measure proteoforms and protein complexes in complex samples, achieving high sensitivity and resolution.

This paper demonstrates how electron capture charge reduction (ECCR) combined with surface induced dissociation improves the characterization of protein complex topology and glycoprotein heterogeneity.

The paper develops a multiparametric pipeline to isolate and characterize adipose-derived extracellular vesicles from human biospecimens, identifying cell-specific biomarkers and pathways associated with obesity.

This study investigates how gut microbiome carbon and sulfur metabolisms support Salmonella during infection by analyzing multi-omics data from infected mice.

The paper investigates the tunable hetero-assembly of a plant pseudoenzyme-enzyme complex, revealing variable stoichiometry and structural insights into their interactions.

The paper introduces a computational method for designing rigid protein assemblies through three-body fusion and validates it experimentally with mass spectrometry and cryo-EM.

This study successfully designs transmembrane beta-barrels from first principles, revealing structural constraints and enabling custom pore design for biotechnology applications.

The paper shows the exon junction complex (EJC) undergoes a compositional switch from an SR-rich to an SR-devoid form, altering mRNP structure and NMD activity.