Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization
Rhonda L. Bacher, Li‐Fang Chu, Cara Argus, Jennifer M. Bolin, Parker Knight, James Alexander Thomson, Ron M. Stewart, Christina M. Kendziorski
This study shows that equalizing cDNA library concentrations before pooling improves gene detection rates and reduces technical artifacts in single-cell RNA-seq data.
Nucleic Acids Research · Oct 20, 2021Peer-reviewedGenomics