Hao Wang

This study adapts Edman degradation and amino acid recognition to expansion microscopy gels to demonstrate in situ protein sequencing principles using synthetic constructs.

The paper performs a genome-wide CRISPR activation screen to identify genes modulating PrPC abundance, finding 531 genes with significant effects and validating 45 of them.

The paper introduces umExM, a method for dense membrane labeling and expansion microscopy to achieve nanoscale visualization of membranes in tissues, demonstrating resolution down to ~35 nm.

The paper shows mechanical force activates TREK-1 channels via PLD2 and lipid clustering, leading to second messenger signaling and ion flow.

This study shows that astrocyte-derived cholesterol, released via apoE, promotes neuroinflammation by enhancing inflammatory signaling in immune cells.

The paper shows astrocyte-derived cholesterol regulates amyloid precursor protein trafficking to lipid rafts, affecting Aβ and sAPP production in neurons, and disrupting cholesterol synthesis reduces amyloid pathology in mice.

This study identifies lipid rafts and phospholipase D2 (PLD2) as targets of inhaled anesthetics, showing they disrupt rafts to activate TREK-1 channels and induce loss of consciousness.