Émilie Ma

Rad52 Acts as an Assembly Chaperone to Form and Stabilize Rad51 Filaments Through a Large C-Terminus 85-Residue Segment

This study identifies an 85-residue segment of Rad52 that facilitates Rad51 filament formation and stability through structural and functional analyses in yeast.

Rad51 filaments assembled in the absence of the complex formed by the Rad51 paralogs Rad55 and Rad57 are outcompeted by translesion DNA polymerases on UV-induced ssDNA gaps

This study shows the Rad55-Rad57 complex promotes Rad51 filament stability on UV-induced single-strand DNA gaps, counteracting error-prone polymerases to favor error-free homologous recombination.

Rad52 oligomeric N-terminal domain stabilizes Rad51 nucleoprotein filaments and contributes to their protection against Srs2

The paper investigates how Rad52's N-terminal domain stabilizes Rad51 filaments and protects them from Srs2, showing that mutations in this domain reduce toxicity in Srs2-deficient cells.