Raphaël Guérois

This study shows RNA directly interacts with BRAF to regulate MAPK signaling by facilitating RAF complex formation and MEK1/2 phosphorylation.

This study identifies BRK1 and CCDC53 as key subunits in WAVE and WASH complex assembly, showing their interaction with HSBP1 and their role in regulating actin dynamics and cell migration.

The paper investigates the relationship between DNA methylation and Polycomb recruitment at transposable elements in Arabidopsis, finding that DNA methylation can either antagonize or promote H3K27me3 deposition.

The paper engineers high-affinity cross-reactive antibodies with minimal mutations using deep learning and deep mutational scanning.

The paper identifies a missing component of the ComB T4SS in H. pylori essential for DNA uptake, showing it is a hexameric ATPase interacting with ComB4.

The paper investigates the integration order of IMEXs in V. cholerae, showing that TLC{Phi} and VGJ{Phi} encode Xer activators that facilitate CTX{Phi} integration, explaining the observed integration order in pandemic lineages.

This study uses Cas12a-based knockout screens to identify genetic interactions in the DNA damage response, revealing synthetic lethal and buffering interactions with therapeutic implications.

This study identifies USP7 as a stabilizer of FAN1, showing its role in DNA crosslink repair and suppression of CAG repeat expansion in Huntington's disease.

This paper introduces qB2H, an optimized bacterial two-hybrid system for quantitative PPI analysis, demonstrating improved accuracy and utility in interface mapping and binder optimization.

The paper shows that MBD4 and PMS1-dependent non-canonical MMR work together to repair 5mC deamination, contributing to genome stability.

This study investigates the minimal NHEJ system in M. tuberculosis using structural simulations, assays, and single-molecule analysis, revealing Ku-LigD interactions and their impact on enzymatic activity.

This study identifies a tripartite protein complex essential for ssDNA uptake during natural transformation in firmicutes, validated through structural modeling and experimental assays.

This study identifies CARM1-mediated methylation of ALIX as critical for its interactions with SH3-domain proteins during cytokinesis.

The paper introduces ProTiler-Mut, a framework for analyzing tiling mutagenesis screens to uncover mutation-phenotype linkages, revealing new insights into functional mutation categories and protein interactions.

This study identifies a protein-protein interaction between ComM and DprA that is critical for DprA to recruit ComM to promote recombination during natural transformation in Gram-negative bacteria.

This study identifies an 85-residue segment of Rad52 that facilitates Rad51 filament formation and stability through structural and functional analyses in yeast.

The paper demonstrates that AlphaFold2-Multimer can identify interaction sites in intrinsically disordered regions with improved accuracy using fragment-based approaches and evolutionary information integration.

The paper identifies FANCC as an anti-CO gene conserved from vertebrates to plants and shows it regulates meiotic recombination with FANCE and FANCF.

This paper shows Zip4 directly interacts with Ecm11 to couple crossover formation to synaptonemal complex assembly, preventing excess crossovers.

The paper investigates how Rad52's N-terminal domain stabilizes Rad51 filaments and protects them from Srs2, showing that mutations in this domain reduce toxicity in Srs2-deficient cells.

This study shows that Sir3 promotes NHEJ by inhibiting Sae2-dependent resection through physical interaction, independent of its heterochromatin function.

The paper improves protein-protein interface scoring by integrating atomic-level evolutionary information, achieving higher success rates in docking discrimination.

The paper shows that the Pif1 helicase is inhibited by the Mer3-MutLβ complex during meiotic recombination, limiting gene conversion tract length.

The paper identifies the role of the MutLγ-Exo1 complex in meiotic crossover formation by showing Exo1 recruits Cdc5 to activate MutLγ and highlights its spatial regulation near DSB hotspots.

The paper identifies FLIP as a novel partner of FIGL1 that forms a conserved complex regulating meiotic homologous recombination in Arabidopsis and humans.

This paper identifies three separable functions of Spp1 in meiotic DSB formation, showing it interacts with Set1 and Mer2 complexes to promote recombination initiation.