The paper develops a near-infrared aptamer:dye system for live-cell super-resolution RNA imaging through evolutionary approaches and chemical optimization.

bioRxiv · Sep 18, 2025 · PreprintBiochemistry

Dynamics of poly(A) tail length and non-A residues during the human oocyte-to-embryo transition

Yusheng Liu, Keliang Wu, Fanghong Shao, Hu Nie, Jing‐ye Zhang, Cheng Li, Zhenzhen Hou, Jiaqiang Wang, Bing Zhou, Han Zhao, Falong Lu

The paper profiles poly(A) tail dynamics in human oocytes and embryos, revealing dynamic changes in length and non-A residues, and identifies mechanisms for their regulation.

bioRxiv · Aug 29, 2021 · PreprintDevelopmental Biology

FREQUENT GENE CONVERSION IN HUMAN EMBRYOS INDUCED BY DOUBLE STRAND BREAKS

Dan Liang, Nuria Martí Gutiérrez, Tailai Chen, Yeonmi Lee, Sang-Wook Park, Hong Ma, Amy Koski, Riffat Ahmed, Hayley Darby, Ying Li, Crystal Van Dyken, Aleksei E. Mikhalchenko, Thanasup Gonmanee, Tomonari Hayama, Han Zhao, , Shoukhrat M. Mitalipov

This study shows that gene conversion and NHEJ are major DNA repair mechanisms in human embryos, with gene conversion potentially useful for gene correction but also causing extensive loss of heterozygosity.

bioRxiv · Jun 19, 2020 · PreprintGenetics