Keliang Wu

ANCHOR: haplotype-aware allelic and isoform inference from single-cell long-read RNA sequencing with de novo variant calling

ANCHOR improves variant calling and allele-specific expression analysis in single-cell long-read RNA-seq by integrating haplotype-aware methods and beta-binomial modeling.

Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes

This study identifies impaired maternal mRNA deadenylation as a molecular defect in in vitro matured mouse and human oocytes, leading to abnormal gene expression.

Dynamics of poly(A) tail length and non-A residues during the human oocyte-to-embryo transition

The paper profiles poly(A) tail dynamics in human oocytes and embryos, revealing dynamic changes in length and non-A residues, and identifies mechanisms for their regulation.

Mechanism of cell polarisation and first lineage segregation in the human embryo

This study identifies the mechanism of first lineage segregation in human embryos through cell polarisation, showing it occurs at the 8-cell stage via F-actin and Par complex polarisation, with PLC activation and GATA3 regulation.

FREQUENT GENE CONVERSION IN HUMAN EMBRYOS INDUCED BY DOUBLE STRAND BREAKS

This study shows that gene conversion and NHEJ are major DNA repair mechanisms in human embryos, with gene conversion potentially useful for gene correction but also causing extensive loss of heterozygosity.