Falong Lu

This study identifies impaired maternal mRNA deadenylation as a molecular defect in in vitro matured mouse and human oocytes, leading to abnormal gene expression.

A spatial 3D genomics approach was developed to analyze hierarchical chromatin conformation in situ in tissue sections, revealing organ-specific and conserved chromatin interactions.

The paper introduces eNet, a method to build enhancer networks from single-cell data, showing complex networks regulate cell identity and disease genes better than existing models.

This study shows that non-A residues in poly(A) tails enhance mRNA translation efficiency in mouse and human cells, offering a new approach to improve mRNA-based therapeutics and vaccines.

The paper identifies re-polyadenylation of maternal mRNA degradation intermediates during mammalian oocyte-to-embryo transition using PAIso-seq data across species.

The paper studies mitochondrial mRNA abundance and poly(A) tail dynamics during the oocyte-to-embryo transition in multiple species, finding stable poly(A) tail length patterns and tissue-specific abundance changes.

This study identifies the dynamic roles of non-A residues in poly(A) tails during mouse oocyte-to-embryo transition, showing their impact on mRNA stability and degradation.

This study shows mitochondrial mRNA is a stable reference for RNA-seq normalization, outperforming exogenous spike-ins and reducing batch effects.

The paper introduces a new method to analyze poly(A) tail composition and length, revealing conserved and variable features across eukaryotic species.

This study compares poly(A) tail dynamics during oocyte-to-embryo transition across four mammalian species, identifying conserved and divergent features of maternal mRNA regulation.

The paper quantifies poly(A) tail length dynamics during the mammalian oocyte-to-embryo transition and shows their role in maternal gene expression across species.

The paper introduces mRNA degradation intermediates as a simple indicator of mRNA and cell metabolism, validated in mouse and human cell lines and oocytes.

The paper profiles poly(A) tail dynamics in human oocytes and embryos, revealing dynamic changes in length and non-A residues, and identifies mechanisms for their regulation.

The paper introduces MePAIso-seq2, a method to study m6A, alternative splicing, and poly(A) tailing, finding m6A affects splicing but not polyadenylation site choice and is associated with longer poly(A) tails.