Pervasive head-to-tail insertions of DNA templates mask desired CRISPR/Cas9-mediated genome editing events
Boris V. Skryabin, Delf-Magnus Kummerfeld, Leonid Gubar, Birte Seeger, Helena Kaiser, Anja Stegemann, Johannes Roth, Sven Guenther Meuth, Hermann Josef Pavenstädt, J. Sherwood, Thomas Pap, Roland Wedlich‐Söldner, Cord H. Sunderkötter, Yuri B. Schwartz, Juergen Brosius, Timofey S. Rozhdestvensky
The paper identifies head-to-tail DNA template insertions in CRISPR/Cas9 editing and highlights the failure of PCR to detect them, advocating for comprehensive analysis.
Science Advances · Feb 12, 2020Peer-reviewedGenetics