Author

Leonid Gubar

University of Münster

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Genetics· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:experimental assay· 2 Organism:mouse· 2 Research Focus:variant calling· 2 Research Focus:drug response / drug discovery· 1 Research Focus:gene regulatory network inference· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Anja Stegemann· 2 shared Birte SeegeriD· 2 shared Boris V. SkryabiniD· 2 shared Helena Kaiser· 2 shared Hermann Josef PavenstädtiD· 2 shared Timofey S. RozhdestvenskyiD· 2 shared Cord H. SunderkötteriD· 1 shared Daniela Anne BrauniD· 1 shared

Activity by Year

2020-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 42 mutual co-author links

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2 shared papersMolecular Biology

Anja Stegemann

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Author profile →

2 papers

Recent·Most cited

CRISPR-Cas9 HDR Optimization: RAD52, Denatured and 5'-Modified DNA Templates in Knock-In Mice Generation

Boris V. Skryabin, Daniela Anne Braun, Helena Kaiser, Leonid Gubar, Birte Seeger, Tasneem Khanam, Anja Stegemann, Hermann Josef Pavenstädt, Timofey S. Rozhdestvensky

This study identifies strategies to improve CRISPR-Cas9 HDR efficiency in generating conditional knockout mice by optimizing DNA templates and using RAD52 protein.

iScience · Oct 21, 2025Peer-reviewedMolecular Biology

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR/Cas9-mediated genome editing events

Boris V. Skryabin, Delf-Magnus Kummerfeld, Leonid Gubar, Birte Seeger, Helena Kaiser, Anja Stegemann, Johannes Roth, Sven Guenther Meuth, Hermann Josef Pavenstädt, J. Sherwood, Thomas Pap, Roland Wedlich‐Söldner, Cord H. Sunderkötter, Yuri B. Schwartz, Juergen Brosius, Timofey S. Rozhdestvensky

The paper identifies head-to-tail DNA template insertions in CRISPR/Cas9 editing and highlights the failure of PCR to detect them, advocating for comprehensive analysis.

Science Advances · Feb 12, 2020Peer-reviewedGenetics