This study demonstrates that dual-target siRNA delivery using CD-targeting micelles reduces cyst growth in ADPKD models by silencing TMEM16A and MCP-1.

bioRxiv · Jul 21, 2026 · PreprintBioengineering

Collecting Duct-Targeted Lipid Nanoparticles Deliver Pkd2 mRNA to Restore Polycystin-2 and Attenuate ADPKD

Joshua Giblin, Karla Lambaren, Veronica LaMastro, Isabella Suzuki, Yi Huang, Rowan Simon, Jose Zarate‐Diaz, Ali Osouli, Jessica Pham, Kenneth R. Hallows, Eun Ji Chung

This study develops lipid nanoparticles targeting collecting duct cells to deliver Pkd2 mRNA, restoring polycystin-2 and reducing cysts in ADPKD models.

bioRxiv · Jun 5, 2026 · PreprintBioengineering

Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids

Biao Huang, Zipeng Zeng, Hui Li, Zexu Li, Xi Chen, Jinjin Guo, Chennan C. Zhang, Megan E. Schreiber, Ariel C. Vonk, Tianyuan Xiang, Tadrushi Patel, Yidan Li, Riana K. Parvez, Bálint Dér, Jyun Hao Chen, , Zhongwei Li

This paper develops a synthetic niche to expand nephron progenitor cells, enabling organoid generation and CRISPR screening for kidney development and disease.

bioRxiv · May 25, 2023 · PreprintDevelopmental Biology

Generation of kidney ureteric bud and collecting duct organoids that recapitulate kidney branching morphogenesis

Zipeng Zeng, Biao Huang, Riana K. Parvez, Yidan Li, Jyunhao Chen, Ariel C. Vonk, Matthew Edward Thornton, Tadrushi Patel, Elisabeth A. Rutledge, Albert D. Kim, Jingying Yu, Núria M. Pastor‐Soler, Kenneth R. Hallows, Brendan H. Grubbs, Jill A. McMahon, , Zhongwei Li

The paper generates kidney collecting duct organoids from ureteric bud progenitors and stem cells to study development and disease of the collecting system.

bioRxiv · Apr 27, 2020 · PreprintDevelopmental Biology