Osamu Ohara

A High-Sensitivity and High-Throughput LC-MS Workflow for Low-Input and Single-Cell Proteomics

This paper develops a high-sensitivity and high-throughput LC-MS workflow for low-input and single-cell proteomics, achieving high proteome depth and throughput without specialized equipment.

Serum preprocessing workflows differentially shape biological readout in data-independent acquisition proteomics of systemic juvenile idiopathic arthritis

This study compares serum preprocessing workflows in proteomics to assess their impact on biological readouts in systemic juvenile idiopathic arthritis.

Development of an Ethylenediaminetetraacetic Acid-Enhanced Deep Proteomic Profiling Method for Dried Blood Spots and Its Application in Mouse Disease Models

This paper develops an EDTA-enhanced deep proteomic method for DBS samples, achieving over 7,000 protein identifications and enabling high-throughput analysis in mouse disease models.

Dried blood spot proteomics as a diagnostic framework for citrin deficiency

This paper develops a dried blood spot proteomics method to diagnose citrin deficiency by quantifying SLC25A13 and identifying a 15-protein signature with high accuracy.

TomAP-MS: an improved tomato lectin affinity purification-based mass spectrometry workflow enabling ultra-deep plasma proteomics

The paper improves the TomAP-MS workflow for plasma proteomics using LAcE, increasing protein identifications and throughput while simplifying sample preparation.

RIPK3-RNASE1 axis as a potential therapeutic and clinical monitoring target in VEXAS syndrome

This study identifies the RIPK3-RNASE1 axis as a potential biomarker and therapeutic target in VEXAS syndrome by linking genetic mutations to disease activity and pathological features.

DIA-MS based plasma peptidomic workflow for profiling organ-derived peptides

This paper develops a DIA-MS workflow to identify organ-derived peptides in plasma, finding over 2,000 organ-specific peptides including 19 bioactive ones.

Glial Reactivity and Cognitive Decline Follow Chronic Heterochromatin Loss in Neurons

The paper shows chronic heterochromatin loss in neurons leads to ERV de-repression, inflammation, and cognitive decline in mice.

Thin-diaPASEF: diaPASEF for maximizing proteome coverage in single-shot proteomics

The paper develops Thin-diaPASEF, an optimized diaPASEF method for single-shot proteomics, achieving high protein identification and coverage in HEK cells and multi-sample analyses.

A novel newborn screening modality: Non-targeted proteome analysis using low-cost iron powders

This paper develops a pipeline for non-targeted proteomic analysis of dried blood spots using low-cost iron powders, achieving high throughput and protein identification.

Development of a new genotype-phenotype linked antibody screening system

This paper develops a new antibody screening system using a Golden Gate-based dual-expression vector to rapidly isolate high-affinity cross-reactive antibodies from immunized mice.

Exome-wide benchmark of difficult-to-sequence regions using short-read next-generation DNA sequencing

The paper benchmarks difficult-to-sequence regions in the human exome using machine learning and real-world NGS data from gnomAD to improve clinical sequencing accuracy.