David A. Turner

The paper describes the design and operational considerations of a tissue-engineered skeletal muscle system for microgravity experiments to study muscle atrophy and aging mechanisms.

This study compares the effects of homemade and commercial N2B27 media on mouse gastruloid development, finding differences in timing, cell number, and gene expression patterns.

The paper develops an AI pipeline to automatically segment IMPDH2 rod-ring structures in mouse ESCs, achieving high accuracy and agreement with expert annotations.

The paper identifies a mechanism where Brachyury feedback regulates retinoic acid signaling to drive symmetry-breaking in gastruloids.

Mouse embryonic stem cell aggregates (Gastruloids) form anteroposterior, dorsoventral and left-right axes without extraembryonic tissue or localised signals, through Nodal-initiated, Wnt-stabilised T/Brachyury polarisation.

This study uses Gastruloids to show that Nodal and Wnt signaling interactions drive symmetry breaking and axial organization in early development.