Renier A. L. van der Hoorn

NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana

This study shows that NbRD21 protease regulates receptor-like kinase homeostasis in Nicotiana benthamiana by affecting their post-translational processing and endocytic recycling.

Polychrome activity profiling distinguishes Cys proteases and their isoforms in plants

The paper introduces polychrome activity profiling using fluorescent probes to distinguish Cys proteases and their isoforms in plants by exploiting differential probe affinity.

A membrane-anchored inhibitor of papain-like cysteine proteases promotes Pseudomonas root colonization

This study identifies Cpi1, a membrane-anchored protease inhibitor in Pseudomonas that inhibits plant PLCPs, enhancing bacterial root colonization by modulating immune responses.

Eliminating polyphenol oxidase from Nicotiana benthamiana improves recombinant protein yield and purity and facilitates native-state protein studies

This study shows that eliminating polyphenol oxidase in Nicotiana benthamiana improves recombinant protein yield, purity, and native-state integrity during extraction.

A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen

This study identifies a bacterial outer membrane protein as a substrate of a citrus protease, showing that protease activity reduces pathogen titers and delays disease symptoms in citrus.

Aphid effectors suppress plant immunity via recruiting defence proteins to processing bodies

This study shows aphid effector proteins recruit plant immune regulators to processing bodies, suppressing defenses, while a plant protein counteracts this effect.

Polyphenol oxidase silencing avoids protein crosslinking and enzymatic browning in Nicotiana benthamiana leaf extracts

This study shows that silencing polyphenol oxidase in Nicotiana benthamiana reduces enzymatic browning and protein crosslinking without affecting plant growth.

Reactivity-based proteomics uncovers ATP-induced proteome restructuring

This study uses reactivity proteomics to analyze ATP-induced changes in Arabidopsis proteomes, revealing conformational shifts and metabolite-protein interactions.

Bacterial pathogen deploys iminosugar galactosyrin to manipulate plant glycobiology

This study identifies galactosyrin, an iminosugar produced by Pseudomonas syringae, which inhibits plant β-galactosidase and alters host glycobiology to aid infection.

Immunity gene silencing increases transient protein expression in Nicotiana benthamiana

The paper identifies immunity genes that suppress transient protein expression in Nicotiana benthamiana by silencing them and observing increased GFP expression.

Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplast

The paper shows that the subtilase SBT5.2 cleaves flagellin's flg22 epitope, inactivating its immunogenicity and controlling immune responses in plants.

Extracellular plant subtilases dampen cold shock peptide elicitor levels

The paper shows that the plant subtilase SBT5.2a cleaves the bacterial cold-shock protein csp22, reducing its immunogenicity and pathogen growth.

Using AlphaFold Multimer to discover interkingdom protein-protein interactions

The paper evaluates AlphaFold Multimer's ability to predict interkingdom protein-protein interactions through in-silico screening of pathogen-secreted immune hydrolase inhibitors.

SBT5.2s are the major active extracellular subtilases processing IgG antibody 2F5 in the Nicotiana benthamiana apoplast

The paper shows that SBT5.2 subtilases degrade the IgG antibody 2F5 in Nicotiana benthamiana apoplast, and their knockout increases 2F5 accumulation without affecting plant growth.

Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.

The paper identifies active cytochrome P450s in mouse, plant, and fungal systems using chemical probes and mass spectrometry, showing their subcellular localization and activity-based labeling.

Proteome-wide Profiling of RNA-Binding Protein Responses to flg22 Reveals Novel Components of Plant Immunity

This study identifies flg22-responsive RNA-binding proteins in Arabidopsis, revealing their role in plant immunity and uncovering novel immune components.