Yuewen Sheng

This study determines how the I-F3 CRISPR-associated transposase recognizes and pairs transposon ends for transposition using cryo-EM and biochemical analyses.

The paper analyzes depth-dependent structural damage caused by cryo-FIB milling using apoferritin as a reporter, showing damage varies with ion type and molecular target.

The paper presents cryo-EM structures of human spliceosomes showing how SDE2, FAM32A, and Prp18 regulate 3' splice site selection by counteracting distal bias and stabilizing weaker proximal sites.

This study identifies galactosyrin, an iminosugar produced by Pseudomonas syringae, which inhibits plant β-galactosidase and alters host glycobiology to aid infection.

Cryo-ET of 169 nm β-carboxysomes from low-light Synechococcus elongatus reveals ~639 Rubiscos in concentric layers with CcmM bound at RbcL grooves; QconCAT proteomics yields the complete absolute protein stoichiometry.