This paper introduces a correlative imaging method using nano-DESI MSI to map lipids, metabolites, peptides, and proteins in single tissue sections, revealing molecular gradients in mouse pancreatic tissue.

bioRxiv · Sep 4, 2024 · PreprintBiochemistry

Proteome mapping of the human pancreatic islet microenvironment reveals endocrine-exocrine signaling sphere of influence

Sara J.C. Gosline, Marija Veličković, James C. Pino, Le Z. Day, Isaac Kwame Attah, Adam Swensen, Vincent Danna, Camilo Posso, Karin Rodland, Jing Chen, C Matthews, Martha Campbell‐Thompson, Julia Laskin, Kristin Burnum-Johnson, Ying Zhu, Paul Piehowski

The paper develops a spatial proteomics method to map the human pancreatic islet microenvironment and identifies spatial gradients in RNA processing proteins.

Molecular & Cellular Proteomics · Jun 15, 2023Peer-reviewedSystems Biology

The role of islet lipid composition remodeling in regulation of beta-cell death via ADP-ribosyl-acceptor glycohydrolase ARH3 signaling in insulitis

Ernesto Satoshi Nakayasu, Cailin S. Deiter, Jennifer E Kyle, Michelle A. Guney, Dylan Sarbaugh, Ruichuan Yin, Yi Cui, Carrie Nicora, Farooq Syed, Jonas Juan-Mateu, Raghavendra G. Mirmira, Carmella Evans‐Molina, Decio Laks Eizirik, Bobbie‐Jo Webb‐Robertson, Kristin Burnum-Johnson, , Charles K. Ansong

The paper investigates lipid composition changes in insulitis models and identifies ARH3 as a regulator of beta-cell apoptosis through PLA2G6 signaling.

bioRxiv · Mar 23, 2020 · PreprintSystems Biology