Simultaneous single-cell CRISPR, RNA, and ATAC-seq enables multiomic CRISPR screens to identify gene regulatory relationships
Kaivalya Shevade, Yeqing Angela Yang, Kevin Feng, Karl Mader, Volkan Sevi̇m, Jacob Parsons, Gunisha Arora, Hasnaa A. Elfawy, Rachel Mace, Scot Federman, Rustam Esanov, Shawn Shafer, Eric D. Chow, Laralynne Przybyla
The paper introduces CAT-ATAC, a method combining CRISPR, RNA, and ATAC-seq in single cells to identify gene regulatory relationships and validate them with loss-of-function experiments.
bioRxiv · Feb 11, 2025 · PreprintGenomics