Alan M. Johnson

This paper characterizes SRLase1, an RNase toxin that cleaves the sarcin-ricin loop of 23S rRNA by repurposing EF-Tu, revealing a new mechanism of ribosome inhibition.

This study shows that Reh1 is required for the degradation of 25S rRNA with mutations in the catalytic center, but not for other defective rRNAs.

This study identifies Rei1 and Reh1 as facilitators of eL24 loading onto pre-60S ribosomal subunits in yeast.

The paper identifies a cytoplasmic surveillance pathway that detects and degrades faulty ribosomes during biogenesis but allows defective ribosomes to escape into translation if they bypass the pathway.

The paper shows Reh1 remains on 60S subunits during early translation elongation and is displaced by the growing peptide chain.

The paper shows that a single 2'-O-methylation of ribosomal RNA is essential for ribosome assembly and nuclear export, with Nog2 interacting specifically with the methylated base.

This study identifies Rio2 as a factor that displaces Bud23 from pre-40S ribosomal particles, revealing a mechanism for Bud23 release during ribosome biogenesis in yeast.

This study identifies Bud23's role in promoting SSU Processome progression by linking it to a network of factors that facilitate structural rearrangements during ribosome biogenesis.

The paper shows that the L1 stalk is essential for efficient export of pre-60S ribosomal subunits in yeast, despite previous findings suggesting otherwise.

The paper identifies Tsr4 as a cytoplasmic chaperone for the ribosomal protein Rps2 in yeast, showing Tsr4's role in Rps2 solubility and its absence from nuclear pre-40S particles.

The paper investigates how Utp14 interacts with the SSU Processome and its role in regulating Dhr1 activity and RNA exosome recruitment during ribosome biogenesis.