Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols
Björn D. M. Bean, Colleen J. Mulvihill, Riddhiman K. Garge, Daniel R. Boutz, Olivier Rousseau, Brendan M. Floyd, William Cheney, Elizabeth C. Gardner, Andrew D. Ellington, Edward M. Marcotte, Jimmy Gollihar, Malcolm S. Whiteway, Vincent J. J. Martin
This study engineers yeast to produce cholesterol, enabling functional expression of human opioid and other GPCRs, creating biosensors with measurable agonist responses and antagonist inhibition.
Nature Communications · May 24, 2022Peer-reviewedSynthetic Biology