Vincent J. J. Martin

This study uses biosensor-guided CRISPR screens in yeast to identify genetic targets that enhance production of cis,cis-muconic acid and protocatechuic acid.

The paper improves GPCR biosensors in yeast using a new platform called HERO, achieving significant sensitivity gains through directed evolution.

The paper uses a trifunctional CRISPR screen to identify gene perturbations that enhance fumaric acid tolerance and production in Kluyveromyces marxianus.

This study sequences and annotates the genome of Kluyveromyces marxianus Y-1190, identifies genetic factors for lactose metabolism, and develops tools for genetic manipulation to enhance its use in industrial biotechnology.

This paper characterizes the PcaR transcription factor, solving its crystal structures and demonstrating its function as a dicarboxylic acid biosensor in yeast.

CRAPS enables in vivo assembly of multiple pathway configurations by using yeast chromosomal repair and gRNA activation, resulting in diverse carotenoid-producing strains.

This paper engineers a TetR-type repressor to recognize multiple aromatic inducers and demonstrates its use in both prokaryotic and eukaryotic systems.

This paper improves the microbial synthesis of benzylisoquinoline alkaloids in yeast by using human monoamine oxidase A to increase selectivity and yield of (S)-reticuline and its derivatives.

The paper screens non-conventional yeasts for organic acid tolerance and engineers Pichia occidentalis to produce cis,cis-muconic acid with improved metrics compared to Saccharomyces cerevisiae.

This study elucidates the biosynthesis of unique benzylisoquinoline alkaloids in sacred lotus and enables their microbial synthesis.

The paper introduces a CRISPR-Cas9 toolkit for markerless yeast gene localization and overexpression, enabling efficient genomic modifications and subcellular compartment labeling.

This study engineers yeast to produce cholesterol, enabling functional expression of human opioid and other GPCRs, creating biosensors with measurable agonist responses and antagonist inhibition.

This paper describes a platform for de novo synthesis of nepetalactone in yeast, achieving 3.10 mg/L/OD600 from simple sugars by optimizing pathway genes and deleting competing enzymes.

This paper describes a yeast platform that significantly increases the production of tetrahydroisoquinoline alkaloids, enabling the synthesis of natural and unnatural derivatives.