Malcolm S. Whiteway

The paper improves GPCR biosensors in yeast using a new platform called HERO, achieving significant sensitivity gains through directed evolution.

This study shows that human body temperature increases azole tolerance in Candida albicans by reducing Erg11 degradation via autophagy inhibition.

The paper uses a trifunctional CRISPR screen to identify gene perturbations that enhance fumaric acid tolerance and production in Kluyveromyces marxianus.

This study characterizes ORF19.7608 (PPP1), a biofilm-induced gene in Candida albicans, showing it localizes to punctate structures under biofilm conditions but is not essential for biofilm formation or stress response.

This study sequences and annotates the genome of Kluyveromyces marxianus Y-1190, identifies genetic factors for lactose metabolism, and develops tools for genetic manipulation to enhance its use in industrial biotechnology.

The paper screens non-conventional yeasts for organic acid tolerance and engineers Pichia occidentalis to produce cis,cis-muconic acid with improved metrics compared to Saccharomyces cerevisiae.

This study identifies Adr1 as a transcription factor regulating ergosterol pathway and azole resistance in C. albicans, with functional interactions with Upc2.

The paper introduces a CRISPR-Cas9 toolkit for markerless yeast gene localization and overexpression, enabling efficient genomic modifications and subcellular compartment labeling.

This study engineers yeast to produce cholesterol, enabling functional expression of human opioid and other GPCRs, creating biosensors with measurable agonist responses and antagonist inhibition.

This study profiles single-cell transcriptomes of Candida albicans exposed to antifungal drugs to identify epigenetic responses linked to drug resistance evolution.