Dirk Remus

RNA Polymerase II is a Polar Roadblock to a Progressing DNA Fork

This study investigates the polarity of RNA Polymerase II as a roadblock to DNA replication forks, showing that head-on collisions lead to stable binding and R-loop formation, with TFIIS facilitating hybrid removal.

Cryo-EM structures of G4-stalled CMG reveal inchworm mechanism of DNA translocation

The paper uses cryo-EM to study how G4 DNA structures stall replisomes and reveals an inchworm mechanism for DNA translocation.

A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation

This study identifies a Pold1 hypomorphic mutation in mice that causes reduced embryo size and morphogenetic defects during gastrulation, highlighting the need for coordinated growth and lineage specification.

Multistep loading of PCNA onto DNA by RFC

This study reveals the multistep mechanism of PCNA loading by RFC using cryo-EM, showing open and closed PCNA conformations and a revised model for DNA clamp loading.

Rad53 controls DNA unwinding after helicase-polymerase uncoupling at DNA replication forks

The paper shows that Rad53 kinase limits excessive DNA unwinding at replication forks by controlling CMG helicase activity, preventing fork destabilization.