Jamie A. Hackett

Iterative engineering of a compact Cas9 ortholog for in vivo gene editing via single AAV delivery

The paper engineers a compact Cas9 ortholog, enCme2, for efficient in vivo gene editing via single AAV delivery, showing high specificity and minimal off-target activity.

HDAC3 prevents enhancer hyperactivation to enable developmental transitions

This study identifies HDAC3's role in preventing enhancer overactivation to regulate transcript dosage during developmental transitions.

Embryonic Signatures of Intergenerational Inheritance across Paternal Environments and Genetic Backgrounds

This study examines how paternal environments and genetic backgrounds affect early embryonic development and identifies molecular responses to paternal conditions.

Systematic Epigenome Editing Captures the Context-dependent Instructive Function of Chromatin Modifications

This study uses epigenome editing to systematically analyze how chromatin modifications influence transcription, revealing context-dependent regulatory mechanisms.

Epigenetic Inheritance is Gated by Naive Pluripotency and Dppa2

The paper investigates how epigenetic inheritance is restricted in mammals by showing that naive pluripotency erases heterochromatin domains, but lineage specification allows probabilistic inheritance under favorable conditions.

Dppa2/4 Counteract De Novo Methylation to Establish a Permissive Epigenome for Development

The paper identifies Dppa2/4 as essential for maintaining epigenetic states by counteracting de novo methylation in pluripotent cells, preventing repressive epigenetic memory in lineage-associated genes.

Tracing the Transitions from Pluripotency to Germ Cell Fate with CRISPR Screening

This study identifies key regulators of the transition from pluripotency to germ cell fate using CRISPR screening in PGC-like cells, highlighting Nr5a2 and Zfp296 as critical factors.