Jan Jakub Żylicz

Suspension culture drives metabolic rewiring in human pluripotent stem cells

This study compares 2D and 3D suspension cultures of human pluripotent stem cells, finding metabolic rewiring in 3D cultures with increased glycolysis and altered TCA cycle carbon partitioning.

SCAF1 driven polyadenylation site usage regulates mRNA isoform expression and neuronal differentiation

This study shows that SCAF1 regulates polyadenylation site usage, affecting mRNA isoform expression and neuronal differentiation in mouse embryonic stem cells.

HDAC3 prevents enhancer hyperactivation to enable developmental transitions

This study identifies HDAC3's role in preventing enhancer overactivation to regulate transcript dosage during developmental transitions.

TCA cycle rewiring underpins implantation and histone acetylation programming

This study reveals that TCA cycle rewiring at implantation supports histone acetylation programming and pluripotency transitions in mouse embryos.

Feeder-free culture of naive human pluripotent stem cells retaining embryonic, extraembryonic and blastoid generation potential

This study develops a feeder-free serum-coated system for culturing naive human pluripotent stem cells, maintaining their developmental potential and reducing contamination and mutations.

Tracking H3K27me3 and H4K20me1 during XCI reveals similarities in enrichment dynamics

The paper tracks H3K27me3 and H4K20me1 dynamics during XCI and finds they accumulate concurrently but have distinct genomic distributions, with H4K20me1 possibly contributing to chromatin compaction.

Exploring the role of Polycomb recruitment in Xist-mediated silencing of the X chromosome in ES cells

This study investigates the role of Polycomb recruitment in Xist-mediated X chromosome silencing in mouse ES cells, finding that silencing can occur independently of Polycomb complexes.