Optimized CRISPR/Cas9 Electroporation and Single Cell Cloning Protocol for Generating Pure Cellular Models in Human Immortalized Myoblasts
Adrien Rihoux, Alexie Gagné, Jean Mezreani, Clémence Gonthier-Cummings, Laura K. Hamilton, Éric Samarut, Martine Tétreault
The paper develops a CRISPR/Cas9 electroporation and single-cell cloning protocol for human myoblasts, achieving high editing success rates and purity.
bioRxiv · Sep 8, 2025 · PreprintCell Biology