Serge Nef

The paper shows that the teleost gonad originates from three embryonic tissues, challenging the idea that pronephric contribution is an amniote innovation.

The paper identifies supporting-like cells as an alternative steroidogenic lineage in mammals, conserved across amniotes, and shows their role in embryonic development and evolution.

This study shows that ovarian cellular diversity arises from spatially organized, transcriptionally heterogeneous coelomic epithelium progenitors in fetal mouse and human ovaries, which are primed toward supporting or steroidogenic identities.

This study identifies NEDD4's role in promoting Sertoli cell proliferation and adult Leydig cell differentiation in murine testis development through PI3K-AKT signaling and steroidogenesis.

This study develops a step-wise in vitro differentiation system from mouse embryonic stem cells into glucocorticoid-producing adrenocortical cells using fibronectin and cAMP activators.

The paper maps the single-cell transcriptome of human fetal gonads to define somatic cell lineage specification during development.

This study uses single-cell transcriptomics to redefine the origin and specification of early adrenogonadal progenitors in mice, showing distinct molecular signatures for adrenal and gonadal fates.

This study identifies bi-allelic CCDC146 mutations as a cause of non-syndromic male infertility in humans and mice, showing its role in sperm flagellum structure and microtubule organization.

This study shows that loss of NR5A1 in Sertoli cells after sex determination leads to their transdifferentiation, anoikis, and testis abnormalities.

The paper shows DLC3/Cv-c is essential for testis development in humans and Drosophila, with the StART domain mediating function independent of the GAP domain.

This study identifies TRIM28's role in preventing female-to-male sex reversal in the adult mouse ovary by maintaining the ovarian pathway through SUMOylation.

The paper identifies and characterizes a previously uncharacterized supporting-like cell lineage in developing mouse gonads, showing its origin, specification, and sex-specific differentiation.

The paper investigates oligogenic inheritance of sperm abnormalities in mice, finding that increasing numbers of heterozygous mutations worsen sperm morphology and motility.

The paper compares transcriptomes of fetal and adult Leydig cells in mice to identify gene expression differences and regulatory mechanisms of steroidogenesis.

The paper uses single-cell RNA sequencing to study gene expression dynamics in mouse germ cells during sex determination, identifying sex-specific regulatory patterns and meiotic gene regulation.

This study identifies Wnt5a as a marker for steroidogenic progenitors that give rise to fetal and adult Leydig cells and peritubular myoid cells in the mouse testis.

The paper identifies ASPHD1 as a key driver of reproductive traits in the 16p11.2 locus using biobank data, Mendelian randomization, and animal models.

This study identifies SNP-dependent mechanisms in inbred mice that confer resistance to transgenerational endocrine disruption caused by DEHP exposure.

The paper uses single-cell RNA sequencing to study sex determination in mouse gonadal somatic cells, identifying early progenitors and sex-specific fate acquisition in different cell lineages.

Time-course single-cell RNA-seq of NR5A1-GFP+ mouse XY gonad cells points to one multipotent somatic progenitor pool that yields Sertoli cells and, progressively, steroidogenic Leydig precursors.