Paul A. Wiggins

A universal regulatory mechanism for prevention of replication restart from RNA:DNA hybrids

The paper identifies a conserved mechanism preventing replication restart from RNA:DNA hybrids in bacteria and mammals.

OmniSegger: A time-lapse image analysis pipeline for bacterial cells

OmniSegger is an updated image analysis pipeline for bacterial cells that improves robustness to cell morphology and supports multiple imaging modalities, providing quantitative cellular analyses.

Noise robustness and metabolic load determine the principles of central dogma regulation

The paper develops a mathematical model to show that fitness maximization leads to protein overabundance, a transcriptional floor, and balanced transcription-translation load.

Protein overabundance is driven by growth robustness

The paper investigates protein overabundance in Acinetobacter baylyi by analyzing proliferation dynamics of essential-gene knockouts, finding that most essential proteins are overabundant and that overabundance increases as expression levels decrease.

The one-message-per-cell-cycle rule: A conserved minimum transcription level for essential genes

The paper identifies a conserved minimum transcription level of one message per cell cycle for essential genes across E. coli, yeast, and human, linking it to noise robustness.

Genome-wide protein-DNA interaction site mapping using a double strand DNA-specific cytosine deaminase

This paper introduces a method called 3D-seq to map DNA-protein interaction sites in vivo using a modified cytosine deaminase, demonstrating its application in Pseudomonas aeruginosa transcription factors.