Employing neutron-encoded monoUbs to study E2/E3 ligase activity and selectivity for assembling Ub chains
Bianca D. M. van Tol, Bjorn R. van Doodewaerd, Guinevere S. M. Lageveen‐Kammeijer, Bas C. Jansen, Joanna Liwocha, Rishov Mukhopadhyay, Manfred Wuhrer, Gerbrand J. van der Heden van Noort, Brenda Arlene Schulman, Paul P. Geurink
The paper develops a mass spectrometry-based assay to screen E2/E3 ligase pairs for their ability to form ubiquitin chains and analyze chain types.
bioRxiv · May 22, 2025 · PreprintBiochemistry