This paper introduces c-SerialED, a method for high-quality protein structure determination using continuous serial electron diffraction, achieving atomic resolution with reduced radiation damage.

bioRxiv · Sep 14, 2025 · PreprintMolecular Biology

Nanobinders for Synaptotagmin 1 enable the analysis of synapticvesicle dynamics in rodent and human models.

Rashi Goel, Kristina Jevdokimenko, Ronja Rehm, Jannik Hentze, Paola Agüi‐Gonzalez, Momchil Ninov, Felix Lange, Agata Witkowska, Svenja Bolz, Francesca Pennacchietti, Martina Damenti, Natalie Kaempf, Vladimir Khayenko, Carles Calatayud, Viveka Nand Malviya, , Eugenio F. Fornasiero

The paper introduces NbLumSyt1 nanobinders for analyzing synaptic vesicle dynamics in rodent and human models with improved precision and versatility.

bioRxiv · Apr 16, 2025 · PreprintNeuroscience

AutoLEI: An XDS-based Graphical User Interface for Automated Real-time and Offline Batch 3D ED/MicroED Data Processing

Lei Wang, Yinlin Chen, Emma Rose Scaletti, Pål Stenmark, Gerhard Höfer, Hongyi Xu, Xiaodong Zou

AutoLEI provides a GUI for automated real-time and offline batch processing of 3D ED/MicroED data, improving efficiency and reducing redundant work.

bioRxiv · Apr 12, 2025 · PreprintMolecular Biology

Massive parallel variant characterization identifies NUDT15 alleles associated with thiopurine toxicity

Chase C. Suiter, Takaya Moriyama, Kenneth A. Matreyek, Wentao Yang, Emma Rose Scaletti, Rina Nishii, Wenjian Yang, Keito Hoshitsuki, Minu Singh, Amita Trehan, Chris Parish, Colton J. Smith, Deepa Bhojwani, Liz Y. P. Yuen, Chi Kong Li, , Jun J.J. Yang

The paper identifies 1,103 deleterious NUDT15 variants using functional assays, improving pharmacogenetics-guided thiopurine treatment by accurately predicting toxicity risk alleles.

bioRxiv · Aug 20, 2019 · PreprintGenomics