Yasuteru Urano

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

This paper develops three topical fluorescence probes for intraoperative detection of peritoneal dissemination in high-grade serous ovarian carcinoma, showing high sensitivity and specificity in clinical and mouse models.

This study develops a blood-based liquid biopsy platform using single-molecule enzyme activity profiling to detect pancreatic ductal adenocarcinoma with high specificity and moderate sensitivity across multiple stages.

This study develops water-soluble fluorogenic probes for single-molecule glycosidase activity profiling in blood, revealing -mannosidase as a potential biomarker for liver injury.

This study develops fluorogenic ProTide probes to profile carboxypeptidase activities, showing elevated levels in pancreatic cancer patients.

This paper develops enzyme activity-based prodrugs for precision oncology, showing reduced toxicity and improved efficacy in tumor models.

The paper develops a bioorthogonal fluorescence probe and engineered enzyme for low-background cancer imaging in mouse models.

This paper introduces a programmable chemical strategy called SCCR for automated synthesis of protease-activatable molecules, demonstrating its application in generating fluorogenic probes and ADC linkers.

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.

This paper introduces a liquid biopsy platform that detects early-stage pancreatic adenocarcinoma by analyzing single-molecule protease/peptidase activity in blood samples.