Charisios D. Tsiairis

This study uses optogenetics to show Hydra tissues integrate Wnt signaling over time, leading to pattern formation through cumulative transcriptional responses.

This study identifies Rac1 as a regulator of the segmentation clock by altering gene expression wave periodicity and somite organization.

This study identifies a transcription factor toggle switch between Zic4 and Gata3 that controls epidermal cell identity in Hydra, showing their mutual antagonism determines cell fate.

This study identifies a mechanochemical feedback loop between Wnt signaling and tissue stretching that drives axis formation in Hydra regeneration.

The paper uses single-cell RNA sequencing in Hydra to show that morphallactic regeneration involves progressive cell fate specification, similar to epimorphic regeneration.

The paper introduces a dual-view light sheet microscope for live imaging of large multicellular systems at single-cell resolution and demonstrates its use on intestinal organoids and gastruloids.

The paper shows Zic4 silencing induces transdifferentiation in Hydra and identifies a Wnt-controlled GRN that regulates cell fate and cell cycle re-entry.

The paper shows Wnt3 expression reflects tissue stretching during Hydra regeneration and can drive regeneration without mechanical input.

The paper introduces a phase-gated and unidirectional coupling mechanism to explain cell synchronization in the presomitic mesoderm through Notch signaling.

The paper characterizes a gene expression oscillator in C. elegans larvae, showing it starts in embryos, arrests transiently, and ceases in adults, operating near a SNIC bifurcation.