Jacob S. Lewis

This study determines cryo-EM structures of bacterial DNA polymerase III complexes to elucidate the mechanism of nucleotide incorporation and proofreading.

The paper reconstitutes the pre-Initiation Complex in yeast to determine how CMGE helicases form and function during DNA replication initiation.

The paper visualizes RRS function in vitro, showing gap formation during replisome passage and varying gap frequency based on strand positioning.

The paper shows that Anillin-dependent actin assembly at PML NBs protects genome stability by shielding under-replicated DNA across cell cycles.