Alessandro Costa

The paper reconstitutes the pre-Initiation Complex in yeast to determine how CMGE helicases form and function during DNA replication initiation.

The paper elucidates how Mcm10 facilitates the separation of converging replicative helicases during DNA replication initiation by stabilizing a structural intermediate.

The paper shows that CDK regulation influences MCM helicase loading at yeast origins, revealing how cell cycle control has shaped origin structure and function.

The paper characterizes human MCM double hexamer (hDH) loading using biochemical reconstitution and cryo-EM, revealing differences from yeast in assembly mechanisms and identifying a novel intermediate.

This study uses cryo-EM and biochemical experiments to reveal the mechanism of DNA entry into the cohesin ring, showing how ATP binding and hydrolysis regulate the process.