Keishi Shintomi

CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation

This study shows CDK1-dependent phosphorylation of NuMA's N-terminus is essential for dynein-dynactin-NuMA complex formation, ensuring accurate chromosome segregation.

Reorganization of DNA loops by competition between condensin I and a linker histone

The paper investigates how condensin I and linker histone H1.8 compete to reorganize DNA loops in a sparkler structure formed in Xenopus egg extracts.

Reconstitution of phospho-regulated mitotic chromatid assembly and disassembly

This study reconstitutes mitotic chromatid assembly and disassembly in vitro, revealing phospho-regulation of condensin I through tIDRs and CAP-H phosphorylation.

Functional interplay between condensin I and topoisomerase IIα in single-molecule DNA compaction

This study shows how condensin I and topoisomerase IIα work together to compact DNA into stable structures at the single-molecule level.

Nuclear assembly in giant unilamellar vesicles encapsulating Xenopus egg extract

This study reconstitutes a nucleus in giant unilamellar vesicles using Xenopus egg extract, demonstrating functional nuclear import and pore complexes.