David M. Garcia

The paper identifies 21 novel pseudouridine sites in S. cerevisiae tRNAs using DRS and BACS, and maps their modifying enzymes.

The paper maps base modifications in yeast mitochondrial tRNAs using Nanopore DRS and tD-seq, identifies enzymes responsible for specific modifications, and reveals interdependent modification circuits.

The paper introduces tRNAZAP, a deep learning method using nanopore ionic current signals to classify tRNA isodecoders, achieving better alignment results than BWA-MEM in E. coli and S. cerevisiae.

The paper uses single-molecule techniques to study tRNA structural dynamics before and during interaction with the pseudouridine synthase Pus4, revealing conformational changes and remodeling effects.

This paper identifies a prion, [MIX+], formed by the DNA helicase Mph1/FANCM, which influences genome diversification through DNA damage resistance and meiotic crossover promotion.

This study shows that the prion [BIG+] in yeast increases proliferation but reduces lifespan by altering protein synthesis and translational programs.

This study identifies a prion-based epigenetic mechanism mediated by the DNA helicase Mph1/FANCM that influences genome integrity and promotes phenotypic diversification.