Kristin S. Koutmou

Pseudouridylation landscape across 42 nuclear-encoded S. cerevisiae tRNAs

The paper identifies 21 novel pseudouridine sites in S. cerevisiae tRNAs using DRS and BACS, and maps their modifying enzymes.

Ribosomal Protein bL27 Protects Translating Ribosomes from tmRNA-SmpB

This study identifies bL27's role in preventing tmRNA from interfering with protein synthesis during trans-translation in bacteria.

tRNA isodecoder analysis using Nanopore ionic current signals and deep learning

The paper introduces tRNAZAP, a deep learning method using nanopore ionic current signals to classify tRNA isodecoders, achieving better alignment results than BWA-MEM in E. coli and S. cerevisiae.

Integrating mass spectrometry with Nanopore direct RNA sequencing for de novo modification profiling of bacteriophage MS2

The paper profiles RNA modifications in MS2 bacteriophage using LC-MS/MS and Nanopore DRS, finding {Psi} as the predominant modification.

Mapping the rRNA methylome reveals contributions of methyltransferases to ribosome function and antibiotic sensitivity

This study identifies 17 rRNA methyltransferases in Bacillus subtilis, revealing their roles in ribosome function and antibiotic sensitivity through genetic and biochemical analyses.

RNase 4 improves bottom-up modification mapping of E. coli total tRNAs using HILIC-MS/MS

This paper introduces a method using RNase 4 and HILIC-MS/MS to map tRNA modifications in E. coli with high coverage and without ion-pairing reagents.

The tRNA dihydrouridine synthase DusA has a distinct mechanism in optimizing tRNAs for translation

This study investigates the role of DusA in tRNA modification and translation, finding it selectively enhances specific tRNA charging and translation at certain codons.

TlyA is a 23S and 16S 2'-O-methylcytidine methyltransferase important for ribosome assembly in Bacillus subtilis

This study identifies TlyA as an rRNA methyltransferase critical for ribosome assembly in B. subtilis, showing its role in cold sensitivity and antibiotic resistance.

Cytoplasmic localization of PUS7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance

This study shows that PUS7's cytoplasmic localization enhances stress tolerance by increasing pseudouridylation of mRNAs involved in metal and ROS homeostasis.

A humanized yeast model reveals dominant-negative properties of neuropathy-associated alanyl-tRNA synthetase mutations

The paper demonstrates that neuropathy-associated AARS1 mutations exhibit dominant-negative effects by reducing yeast growth when co-expressed with wild-type AARS1.