Nicole Paczia

This paper presents a coupled strategy for enzyme discovery, evolution, and yeast chassis adaptation to improve microbial biopolymer valorisation, demonstrating it with laccases for lignin depolymerisation.

This study investigates whether NUDT2 loss and subsequent Ap4A accumulation affects the cGAS-STING-TBK1 pathway, finding that physiological levels of Ap4A do not inhibit the pathway.

The paper reconstructs minimal pyrenoids using EPYC1 proteins to study their evolution and carbon concentration mechanisms.

This study investigates flavin redox behavior under prebiotic hydrothermal conditions, showing their versatility in electron transfer with metals and their potential role as electron shuttles in early geochemical systems.

This study shows sex differences in Drosophila intestinal metabolism affect infection outcomes, with males clearing pathogens via antioxidant pathways while females experience higher pathogen virulence.

The paper identifies Form IF Rubiscos that are highly active, specific, and function without the small subunit, challenging the necessity of subunit complexity in Rubisco evolution.

This paper combines adaptive laboratory evolution and machine learning to engineer a methylsuccinate module for synthetic C1 metabolism in E. coli, achieving growth in three weeks versus six months with evolution alone.

This study investigates the role of Tad pili in biofilm formation and surface sensing in Bradyrhizobium diazoefficiens, revealing their regulatory function and connection to c-di-GMP levels.

This paper designs and tests synthetic CO2 fixation pathways in E. coli, demonstrating their potential to outperform natural pathways.

This paper investigates the reduction of NAD and NMN on mineral surfaces under abiotic conditions, showing NAD's specificity and the role of the AMP tail in preventing overreduction.

This study presents the first molecular structure of methylthio-alkane reductase, revealing metalloclusters that inform its function and evolutionary relationship to nitrogenases.

The paper identifies sphingolipids from a bacterial PKS as mediators of microbiota protection against pathogens in C. elegans.

This study identifies the HflK-HflC complex as essential for aerobic respiration in E. coli by regulating IspG abundance and ubiquinone levels.

The paper describes a method to temporarily modulate phage DNA modifications using NgTET, enabling efficient Cas-mediated mutagenesis and functional analysis of phage epigenome.

This paper presents a computational workflow for designing and testing E. coli metabolic sensors that detect glyoxylate and glycolate across a wide concentration range, with applications in bioengineering and environmental monitoring.

This study evolves an engineered microbial community to show how metabolic interactions increase, leading to interdependence and indirect selection for mutualism.

This study shows that MprF in Lactiplantibacillus plantarum helps it survive in Drosophila gut during inflammation by reducing immune response and maintaining microbial stability.

This study identifies multiple transcriptional regulators controlling glycolate metabolism in Paracoccus denitrificans, revealing a complex regulatory network for carbon assimilation.

This study establishes Chlamydomonas reinhardtii as a platform for chloroplast synthetic biology, enabling high-throughput engineering of plastomes and demonstrating improved biomass production through synthetic pathways.

This study identifies NAD-capped RNAs during T4 phage infection of E. coli, showing dynamic changes in NAD-capping and revealing a phage-encoded Nudix hydrolase involved in NAD-RNA decapping.

This paper develops an in vitro transcription-based biosensing workflow to quantify glycolate production in the CETCH cycle, showing high correlation with LC-MS at lower cost and faster speed.

This study constructs a metabolic and genetic linked in vitro network that generates its own building blocks from non-living matter and demonstrates life-like behavior.

This study replaces the glycerate pathway with the β-hydroxyaspartate cycle in Pseudomonas putida, resulting in faster growth and higher biomass yield on ethylene glycol.

The paper identifies NAD-RNAs and ADPR-RNA decapping in archaea, showing NAD-RNAs are prevalent in Sulfolobus acidocaldarius and Haloferax volcanii, and Saci_NudT5 hydrolyzes ADPR-RNA.

The paper introduces METIS, an active learning workflow for optimizing genetic and metabolic networks, demonstrating significant improvements in efficiency with minimal experiments.