John F.X. Diffley

The paper reconstitutes the pre-Initiation Complex in yeast to determine how CMGE helicases form and function during DNA replication initiation.

This paper develops a method to selectively kill cancer cells with TP53 mutations by using RNA-guided Cas12a2 to induce chromatin shredding through targeting cancer-specific transcripts.

The paper elucidates how Mcm10 facilitates the separation of converging replicative helicases during DNA replication initiation by stabilizing a structural intermediate.

This study reconstitutes sister chromatid cohesion in budding yeast, revealing two pathways for cohesin recruitment and a two-step mechanism for co-entrapment.

This study directly visualizes replisome-cohesin interactions and shows that replisomes pass through cohesin rings, establishing cohesion between sister chromatids.

This study reveals DNA replication fork stabilization and restart mechanisms using biochemical reconstitution in budding yeast.

This study identifies how the DNA damage checkpoint stabilizes replication forks by preventing excess origin firing and fork collapse through the regulation of PCNA and HLTF.

The paper introduces CRISPR-Taiji to engineer chromosome-specific aneuploidy and characterizes its transcriptomic effects, linking aneuploidy to ccRCC phenotypes.

The paper characterizes human MCM double hexamer (hDH) loading using biochemical reconstitution and cryo-EM, revealing differences from yeast in assembly mechanisms and identifying a novel intermediate.