Evan M. Cornett

This study identifies changes in non-histone lysine methylation during neuronal differentiation of LUHMES cells using quantitative proteomics.

The paper quantifies non-histone lysine methylation sites and lysine demethylases in breast cancer cell lines using TMT labeling without antibody enrichment, identifying differentially abundant sites and enzymes.

The paper introduces a quantitative framework for ChIP-seq analysis that avoids the need for spike-in reagents by leveraging competitive binding during the IP step and provides a visualization technique for richer data characterization.

This study compares monoclonal and polyclonal antibodies for ChIP-seq of histone modifications, finding monoclonals perform similarly in most cases but show differences for H3K27ac.