Gary H. Karpen

The paper uses FOC-Map to analyze heterochromatin replication in Drosophila, finding replication foci initially at the PCH domain boundary and later within low-HP1a regions, with CDC45 depletion from the domain.

This study shows that ORC interacts with HP1a via specific motifs to regulate heterochromatin structure and function in Drosophila.

This study shows HP1a promotes chromatin liquidity and drives spontaneous heterochromatin compartmentalization through interfacial tension modulation.

The paper identifies an expanded HP1a-binding motif, HACs, and shows their role in heterochromatin assembly through molecular interactions and simulations.

The paper introduces DiMeLo-seq, a method for mapping protein-DNA interactions using long-read sequencing, and provides a protocol and analysis tool.

The paper presents a comprehensive analysis of fully assembled human centromeres, revealing their architecture, variation, and evolutionary dynamics at high resolution.

The paper identifies genomic associations with ex vivo DNA damage responses to ionizing radiation in mice, highlighting pathway differences across radiation types and timing.

The paper shows that evolutionarily dynamic ZAD-ZNF genes in Drosophila are more likely to be essential, with Nicknack being necessary for larval development despite its rapid evolution.

The paper investigates the 3D organization of Drosophila melanogaster pericentromeric heterochromatin domains and their spatial interactions with euchromatic regions enriched in H3K9me2/3 marks.

The paper identifies large-scale haplotypes spanning centromeric regions in humans, revealing deep diversity including Neanderthal introgressions and ancient African lineages.