Michael Knop

This paper introduces a chromosomally integrated yeast surface display system for scalable, quantitative antibody profiling and serological assays.

CODIAC profiles spatial protein localization and abundance changes using image-enabled sorting and machine learning, detecting perturbation effects on the spatial proteome.

The paper improves CRISPR-Cas12a-assisted gene tagging in mammalian cells by enhancing HDR efficiency and reducing off-target integrations through modified cassettes and repair pathway modulation.

This study identifies ORF2 as a key HEV protein that suppresses antiviral responses in hepatocytes, enabling viral persistence.

The paper shows that Saccharomycodes ludwigii undergoes meiosis without recombination, relying on frequent fertilization within tetrads instead.

This study investigates the roles of NoxA and Yap1 in redox homeostasis and pathogenicity of Verticillium dahliae, showing their essential functions in development, stress tolerance, and antioxidant gene regulation.

This study shows that starvation promotes frequent nonself fusion in V. dahliae, enabling parasexual interactions despite allorecognition systems, with autophagy protecting incompatible heterokaryons.

The paper investigates the distinct physiological roles of NatA and NatB in S. cerevisiae through transcriptome, translatome, and proteome profiling of mutants, revealing specific effects on gene expression and protein folding.

The paper introduces CASTLING, a method for efficiently creating pooled yeast gene tagging libraries using CRISPR/Cas12a and recombineering, achieving high tagging success rates.

The paper uses a fluorescent timer to analyze Bicoid gradient dynamics in Drosophila embryos, revealing parameters consistent with the synthesis-diffusion-degradation model.

The paper describes a C-SWAT library for high-throughput tagging of yeast ORFs and validates it by tagging the yeast proteome with fluorescent proteins.

The paper characterizes the MAL32 promoter in S. cerevisiae, showing its inducibility by maltose and distinct kinetics compared to the GAL1 promoter, and provides tools for its use.

This study evaluates how green fluorescent protein (greenFP) maturation kinetics and proteasomal degradation affect tandem fluorescent protein timers (tFTs), identifying greenFPs that degrade efficiently and guidelines for tFT design.