Andrew D. Hanson

This paper investigates suicide Thi4 thiazole synthases, showing genomic evidence suggesting non-suicidal function and supporting this with functional experiments in E. coli.

This study uses directed evolution to extend the lifespan of an Arabidopsis enzyme by increasing its abundance and improving its resistance to a toxic intermediate.

The paper shows that synonymous mutations, not nonsynonymous ones, drove growth improvements in bacterial THI4 enzymes adapted for plant use through continuous directed evolution.

This study improves the aerotolerance and activity of sulfide-dependent THI4 enzymes through directed evolution in yeast, identifying beneficial mutations that enhance their performance under aerobic conditions.

This study uses continuous directed evolution to engineer feedback-resistant Arabidopsis arogenate dehydratase in a microbial platform, achieving resistance comparable to a known variant.

The paper describes a method to improve enzymes for plant applications using continuous directed evolution in yeast, demonstrating its effectiveness with a bacterial thiamin synthesis enzyme.

The paper identifies aerotolerant, multiple-turnover THI4 thiazole synthases in aerobic prokaryotes and shows they use cobalt as a cofactor instead of iron.

The paper discusses two systems for continuous directed evolution of enzymes and tests their adaptation for plant enzyme engineering.